SYNCHRONOUS FLUORESCENCE SPECTROSCOPIC, IMMUNOAFFINITY CHROMATOGRAPHIC AND P-32 POSTLABELING ANALYSIS OF HUMAN PLACENTAL DNA KNOWN TO CONTAIN BENZO[A]PYRENE DIOL EPOXIDE ADDUCTS

被引：55
作者：
MANCHESTER, DK
WILSON, VL
HSU, IC
CHOI, JS
PARKER, NB
MANN, DL
WESTON, A
HARRIS, CC
机构：
[1] NCI,DIV CANC ETIOL,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892
[2] CHILDRENS HOSP,KEMPE RES CTR,DENVER,CO 80218
[3] UNIV COLORADO,HLTH SCI CTR,DEPT PEDIAT,DENVER,CO 80262
[4] UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL,DENVER,CO 80262
[5] UNIV MARYLAND,BALTIMORE,MD 21201
来源：
CARCINOGENESIS | 1990年 / 11卷 / 04期
关键词：
D O I：
10.1093/carcin/11.4.553
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Human placenta readily catalyzes the biotransfonnation of polycyclic aromatic hydrocarbons (PAHs) and other carcinogens to reactive metabolites that can damage DNA through formation of covalent adducts. Placenta is widely available for epidemiologic studies and may be a useful dosimeter for carcinogen exposures In humans. However, previous studies of human placental DNA have yielded discrepant results with respect to PAH-DNA adducts. In order to resolve some of the issues surrounding these discrepancies, placental DNA samples known to contain benzo[a]pyrene diol epoxide adducts were also analyzed by 32P-pastlabeling and immunmnity chromatography. Results indicate that previous discrepancies can be accounted for by methodologic factors affecting the spcificities of adduct assays in biological samples and suggest that human placental DNA contains adducts derived from multiple PAHs. © 1990 Oxford University Press.
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页码：553 / 559
页数：7
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