NISIN AS A MODEL FOOD PRESERVATIVE

被引:92
作者
HANSEN, JN
机构
[1] Department of Chemistry and Biochemistry, College of Life Sciences, Chemistry Building 091, University of Maryland, College Park
关键词
NISIN; SUBTILIN; LANTIBIOTICS; FOOD PRESERVATIVES;
D O I
10.1080/10408399409527650
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Nisin is a ribosomally synthesized peptide that has broad-spectrum antibacterial activity, including activity against many bacteria that are food-spoilage pathogens. Nisin is produced as a fermentation product of a food-grade bacterium, and the safety and efficacy of nisin as a food preservative have resulted in its widespread use throughout the world, including the U.S. Nisin is a member of the class of antimicrobial substances known as lantibiotics, so called because they contain the unusual amino acid lanthionine. Lantibiotics, in general, have considerable promise as food preservatives, although only nisin has been sufficiently well characterized to be used for this purpose. As the number of known natural lantibiotics has increased and their useful characteristics have been explored, it has become desirable to synthesize structural analogs of nisin and other lantibiotics that do not occur naturally. The fact that lantibiotics are gene-encoded peptides synthesized by transcription and translation allows structural variants to be generated by mutagenesis. This review focuses on the progress that has been made in the construction and biological expression of genetically engineered nisin structural analogs. For example, a host-vector pair has been engineered that permits the construction of mutants of the structural gene for subtilin, which is a naturally occurring structural analog of nisin. The vector is designed in such a way that the mutant gene can be substituted for the natural subtilin gene in the chromosome of Bacillus subtilis, which in turn directs the transcription, translation, posttranslational modifications, and secretion of the mature form of the structural analog. Several structural analogs have been constructed, and their properties have provided insight into some of the structure-function relationships in lantibiotics, as well as their mechanism of antimicrobial action. These advances are assessed together with potential problems in the future development of nisin analogs as valuable new food preservatives.
引用
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页码:69 / 93
页数:25
相关论文
共 86 条
  • [1] EPIDERMIN - SEQUENCING OF A HETERODET TETRACYCLIC 21-PEPTIDE AMIDE ANTIBIOTIC
    ALLGAIER, H
    JUNG, G
    WERNER, RG
    SCHNEIDER, U
    ZAHNER, H
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 160 (01): : 9 - 22
  • [2] GENETIC-ANALYSIS OF EPIDERMIN BIOSYNTHETIC GENES AND EPIDERMIN-NEGATIVE MUTANTS OF STAPHYLOCOCCUS-EPIDERMIDIS
    AUGUSTIN, J
    ROSENSTEIN, R
    WIELAND, B
    SCHNEIDER, U
    SCHNELL, N
    ENGELKE, G
    ENTIAN, KD
    GOTZ, F
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 204 (03): : 1149 - 1154
  • [3] BACILLUS-SUBTILIS REQUIRES A STRINGENT SHINE-DALGARNO REGION FOR GENE-EXPRESSION
    BAND, L
    HENNER, DJ
    [J]. DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1984, 3 (01): : 17 - 21
  • [4] BANERJEE S, 1988, J BIOL CHEM, V263, P9508
  • [5] DETECTION AND ACTIVITY OF LACTACIN-B, A BACTERIOCIN PRODUCED BY LACTOBACILLUS-ACIDOPHILUS
    BAREFOOT, SF
    KLAENHAMMER, TR
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 45 (06) : 1808 - 1815
  • [6] BIOCHEMICAL BASIS FOR NITRITE-INHIBITION OF CLOSTRIDIUM-BOTULINUM IN CURED MEAT
    BENEDICT, RC
    [J]. JOURNAL OF FOOD PROTECTION, 1980, 43 (11) : 877 - 891
  • [7] INDUCTION OF AUTOLYSIS OF STAPHYLOCOCCI BY THE BASIC PEPTIDE ANTIBIOTICS PEP-5 AND NISIN AND THEIR INFLUENCE ON THE ACTIVITY OF AUTOLYTIC ENZYMES
    BIERBAUM, G
    SAHL, HG
    [J]. ARCHIVES OF MICROBIOLOGY, 1985, 141 (03) : 249 - 254
  • [8] BLACKBURN P, 1836, Patent No. 2625
  • [9] GENETIC CONSTRUCTION OF NISIN-PRODUCING LACTOCOCCUS-LACTIS SUBSP CREMORIS AND ANALYSIS OF A RAPID METHOD FOR CONJUGATION
    BROADBENT, JR
    KONDO, JK
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (02) : 517 - 524
  • [10] BRON S, 1990, MOL BIOL METHODS BAC, P75