EXPRESSION OF A RECOMBINANT ENTAMOEBA-HISTOLYTICA ANTIGEN IN A SALMONELLA-TYPHIMURIUM VACCINE STRAIN

被引:19
作者
CIESLAK, PR
ZHANG, TH
STANLEY, SL
机构
[1] WASHINGTON UNIV,SCH MED,DEPT MED,ST LOUIS,MO 63110
[2] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110
关键词
ENTAMOEBA-HISTOLYTICA; SALMONELLA-TYPHIMURIUM; RECOMBINANT;
D O I
10.1016/0264-410X(93)90264-X
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The expression of a major surface antigen of the intestinal protozoal parasite Entamoeba histolytica in an attenuated Salmonella typhimurium vaccine strain is described. A polymerase chain reaction fragment derived from cDNA encoding the serine-rich Entamoeba histolytica protein, SREHP, was introduced into S. typhimurium chi3987 (DELTAcya DELTAcrp DELTAasd) using a plasmid expression vector (pYA292) containing the aspartate semialdehyde (asd) gene. S. typhimurium expressing recombinant SREHP as a SREHP/maltose binding protein fusion protein was administered orally to mice and gerbils (an important animal model for E. histolytica infection) and was recovered from splenic tissue in both species. Our study demonstrates the feasibility of expressing recombinant amoebic proteins in attenuated S. typhimurium strains, and shows that vaccine strains of S. typhimurium can successfully infect the gerbil, a widely used model for amoebic liver abscess and intestinal amoebiasis.
引用
收藏
页码:773 / 776
页数:4
相关论文
共 23 条
[1]  
CHADEE K, 1985, AM J PATHOL, V119, P485
[2]   SALMONELLA-TYPHIMURIUM DELETION MUTANTS LACKING ADENYLATE-CYCLASE AND CYCLIC-AMP RECEPTOR PROTEIN ARE AVIRULENT AND IMMUNOGENIC [J].
CURTISS, R ;
KELLY, SM .
INFECTION AND IMMUNITY, 1987, 55 (12) :3035-3043
[3]  
DELEON A, 1970, ARCH INVESTIGACION S, V1, P205
[4]   ORAL VACCINATION OF MICE AGAINST TETANUS BY USE OF A LIVE ATTENUATED SALMONELLA CARRIER [J].
FAIRWEATHER, NF ;
CHATFIELD, SN ;
MAKOFF, AJ ;
STRUGNELL, RA ;
BESTER, J ;
MASKELL, DJ ;
DOUGAN, G .
INFECTION AND IMMUNITY, 1990, 58 (05) :1323-1326
[5]   CLONING AND CHARACTERIZATION OF THE ASD GENE OF SALMONELLA-TYPHIMURIUM - USE IN STABLE MAINTENANCE OF RECOMBINANT PLASMIDS IN SALMONELLA VACCINE STRAINS [J].
GALAN, JE ;
NAKAYAMA, K ;
CURTISS, R .
GENE, 1990, 94 (01) :29-35
[6]  
GUZMAN CA, 1991, INFECT IMMUN, V59, P4391
[7]   MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS [J].
HITCHCOCK, PJ ;
BROWN, TM .
JOURNAL OF BACTERIOLOGY, 1983, 154 (01) :269-277
[8]   ANTIGENS OF ENTAMOEBA-HISTOLYTICA RECOGNIZED BY IMMUNE SERA FROM LIVER-ABSCESS PATIENTS [J].
JOYCE, MP ;
RAVDIN, JI .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1988, 38 (01) :74-80
[9]   TRANSDUCTION OF LINKED GENETIC CHARACTERS OF THE HOST BY BACTERIOPHAGE-PL [J].
LENNOX, ES .
VIROLOGY, 1955, 1 (02) :190-206
[10]   ISOLATION AND CHARACTERIZATION OF GENOMIC CLONES ENCODING A SERINE-RICH ENTAMOEBA-HISTOLYTICA PROTEIN [J].
LI, E ;
KUNZJENKINS, C ;
STANLEY, SL .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 50 (02) :355-358