A NOVEL CALCIUM-DEPENDENT ACTIVATOR OF RETINAL ROD OUTER SEGMENT MEMBRANE GUANYLATE-CYCLASE

被引：53
作者：
POZDNYAKOV, N
YOSHIDA, A
COOPER, NGF
MARGULIS, A
DUDA, T
SHARMA, RK
SITARAMAYYA, A
机构：
[1] OAKLAND UNIV,EYE RES INST,ROCHESTER,MI 48309
[2] UNIV LOUISVILLE,SCH MED,DEPT ANAT SCI & NEUROBIOL,LOUISVILLE,KY 40292
[3] PENN COLL OPTOMETRY,REGULATORY & MOLEC BIOL UNIT,PHILADELPHIA,PA 19141
来源：
BIOCHEMISTRY | 1995年 / 34卷 / 44期
关键词：
D O I：
10.1021/bi00044a002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The membrane guanylate cyclase in retinal rod outer segments (ROS-GC) is known to be negatively regulated by calcium; when the calcium concentration is reduced below the dark-adapted level of about 500 nM, the enzyme is activated by a soluble protein. We now report that the enzyme is also positively regulated by calcium; a novel soluble protein is identified and purified from bovine retina which activates ROS-GC, with half-maximal activation occurring at 2-5 mu M calcium. The activation is dose-dependent, and at its maximum, cyclase is stimulated up to 25-fold. The activator has a molecular mass of about 40 kDa and is a multimer of a 6-7 kDa peptide.
引用
收藏
页码：14279 / 14283
页数：5
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