MANUAL SOLID-PHASE SEQUENCE-ANALYSIS OF POLYPEPTIDES USING 4-N,N-DIMETHYLAMINOAZOBENZENE 4'-ISOTHIOCYANATE

被引：42

作者：

CHANG, JY

机构：

[1] Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 578卷 / 01期

关键词：

Amino acid sequence; Dimethylaminoazobenzene isothiocyanate; Polypeptide; Solid phase analysis;

D O I：

10.1016/0005-2795(79)90126-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A manual solid-phase method for sequence analysis of polypeptides is described. The immobilized polypeptide was subjected to stepwise degradation by Edman-type reagent, using the 4-N,N-dimethylaminoazobenzene 4′-isothiocyanate phenyllisothiocyanate double coupling method. The N-terminal amino acids were released (after conversion reaction) as 4-N,N-dimethylaminoazobenzene 4′-thiohydantoin (identified by thin layer chromatography) and phenylthiohydantoin derivatives. The method required 2-10 nmol polypeptide. © 1979.

引用

页码：188 / 195

页数：8

共 21 条

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[5] 4-NN-DIMETHYLAMINOAZOBENZENE 4'-ISOTHIOCYANATE, - NEW CHROMOPHORIC REAGENT FOR PROTEIN SEQUENCE-ANALYSIS
CHANG, JY
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[7] HIGH-SENSITIVITY SEQUENCE-ANALYSIS OF PEPTIDES AND PROTEINS BY 4-NN-DIMETHYLAMINOAZOBENZENE 4'-ISOTHIOCYANATE
CHANG, JY
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[8] IMPROVED CHROMATOGRAPHIC IDENTIFICATION OF COLORED AMINO-ACID THIOHYDANTOINS
CHANG, JY
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[9] DESTRUCTION OF SERINE AND THREONINE THIOHYDANTOINS DURING THE SEQUENCE DETERMINATION OF PEPTIDES BY 4-N,N-DIMETHYLAMINOAZOBENZENE 4'-ISOTHIOCYANATE
CHANG, JY
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 578 (01) : 175 - 187
[10] NEW APPROACH FOR SOLID-PHASE SEQUENCE DETERMINATION OF PROTEINS
CHANG, JY
CREASER, EH
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