MANUAL SOLID-PHASE SEQUENCE-ANALYSIS OF POLYPEPTIDES USING 4-N,N-DIMETHYLAMINOAZOBENZENE 4'-ISOTHIOCYANATE

被引：42
作者：
CHANG, JY
机构：
[1] Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 578卷 / 01期
关键词：
Amino acid sequence; Dimethylaminoazobenzene isothiocyanate; Polypeptide; Solid phase analysis;
D O I：
10.1016/0005-2795(79)90126-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A manual solid-phase method for sequence analysis of polypeptides is described. The immobilized polypeptide was subjected to stepwise degradation by Edman-type reagent, using the 4-N,N-dimethylaminoazobenzene 4′-isothiocyanate phenyllisothiocyanate double coupling method. The N-terminal amino acids were released (after conversion reaction) as 4-N,N-dimethylaminoazobenzene 4′-thiohydantoin (identified by thin layer chromatography) and phenylthiohydantoin derivatives. The method required 2-10 nmol polypeptide. © 1979.
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页码：188 / 195
页数：8
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