EVIDENCE FOR 2 DISTINCT TYPES OF POSTSYNAPTIC ALPHA-ADRENOCEPTOR IN VASCULAR SMOOTH-MUSCLE INVIVO

The effects of the highly selective α1‐adrenoceptor antagonist, prazosin, and the relatively selective α2‐adrenoceptor antagonist, yohimbine, on the pressor responses to intravenous injections of phenylephrine and noradrenaline have been examined in anaesthetized cats and pithed rats in an attempt to determine whether α1‐ and α2‐adrenoceptors are located postsynaptically on vascular smooth muscle. In anaesthetized cats prazosin caused a much greater reduction in the pressor responses to phenylephrine than to noradrenaline or splanchnic nerve stimulation (after adrenalectomy). Yohimbine was of similar potency in reducing the pressor responses to each stimulus. A differential blocking activity of prazosin against intra‐arterial injections of phenylephrine and noradrenaline was also demonstrated in the blood‐perfused cat hind limb. As in the whole animal, prazosin was more potent against phenylephrine than noradrenaline. A similar, though less marked, effect was seen in the mesenteric circulation, but not in the renal circulation, where prazosin was almost equipotent in reducing responses to phenylephrine and noradrenaline. In pithed rats prazosin was a potent, competitive antagonist of phenylephrine, but had little effect against noradrenaline; only the responses to high doses of noradrenaline were reduced by prazosin. Yohimbine was approximately equipotent as an antagonist of phenylephrine and noradrenaline. In the anococcygeus muscle, prazosin was as potent an antagonist of noradrenaline as it was of phenylephrine on vascular smooth muscle. The results suggest that there are two types of α‐adrenoceptor in the vasculature of cats and rats. Phenylephrine produces pressor responses by stimulating one type of postsynaptic α‐adrenoceptor that is blocked by prazosin and yohimbine; these are α1‐adrenoceptors. Noradrenaline exerts some of its effect via these receptors but most of its effect appears to be exerted through prazosin‐insensitive receptors. The latter receptors appear to differ from α2‐adrenoceptors. 1979 British Pharmacological Society