STOPPED FLOW AND MERGING ZONES - NEW APPROACH TO ENZYMATIC ASSAY BY FLOW INJECTION ANALYSIS

被引：116
作者：
RUZICKA, J
HANSEN, EH
机构：
[1] Chemistry Department A, The Technical University of Denmark, Building 207
来源：
ANALYTICA CHIMICA ACTA | 1979年 / 106卷 / 02期
关键词：
D O I：
10.1016/S0003-2670(01)85004-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Four alternative flow injection methods based on the concept of merging zones have been developed for the assay of glucose in serum with glucose dehydrogenase. Special attention has been paid to the dispersion and synchronization of the merging zones and to methods of measuring the blank value of serum. The optimal procedure, based on rate measurements, allows the assay of glucose to be done at a rate of 100 determinations per hour, with the analytical readout available 30 s after sample injection. The assay requires less than one unit of enzyme per sample. © 1979.
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页码：207 / 224
页数：18
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