A QUANTITATIVE MICROASSAY OF CARBOHYDRATE-MEDIATED CELL-ADHESION TO GLYCOCONJUGATES IMMOBILIZED ON POLYSTYRENE PLATES

被引：6

作者：

DRESSEN, F ^{[1
]}

UHLENBRUCK, G ^{[1
]}

HANISCH, FG ^{[1
]}

机构：

[1] UNIV CLIN COLOGNE, INST IMMUNOBIOL, KERPENER STR 15, W-5000 COLOGNE 41, GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 206卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90380-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microadhesion assay that allows the quantitative determination of carbohydrate-mediated cell adhesion to glycoconjugates immobilized on 96-well polystyrene plates has been developed. After dislodging nonadherent cells by centrifugation, specifically bound cells are quantified by colorimetric analysis of a blue formazan product generated from the dye 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide by enzymatic reduction. Carbohydrate specificity of the cell adhesion was demonstrated by inhibition analyses and the general applicability of the assay was proved with indicator cells of three different origins: mouse fibrosarcoma cells, Chang liver cells, and human breast carcinoma cells (MDA-MB 231). © 1992.

引用

页码：369 / 375

页数：7

共 28 条

[1]

BLACKBURN CC, 1986, J BIOL CHEM, V261, P2873

[2]

BLACKBURN CC, 1983, J BIOL CHEM, V258, P1180

[3] CELL-SURFACE CARBOHYDRATES IN CELL RECOGNITION AND RESPONSE [J].

BRANDLEY, BK ;

SCHNAAR, RL .

JOURNAL OF LEUKOCYTE BIOLOGY, 1986, 40 (01) :97-111

[4] RAPID COLORIMETRIC ASSAY FOR CELL-GROWTH AND SURVIVAL - MODIFICATIONS TO THE TETRAZOLIUM DYE PROCEDURE GIVING IMPROVED SENSITIVITY AND RELIABILITY [J].

DENIZOT, F ;

LANG, R .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 89 (02) :271-277

[5] TUMOR-CELL SURFACE CARBOHYDRATE AND THE METASTATIC PHENOTYPE [J].

DENNIS, JW ;

LAFERTE, S .

CANCER AND METASTASIS REVIEWS, 1987, 5 (03) :185-204

[6]

GUARNACCIA SP, 1982, J BIOL CHEM, V257, P4288

[7] TUMOR-ASSOCIATED CARBOHYDRATE ANTIGENS [J].

HAKOMORI, S .

ANNUAL REVIEW OF IMMUNOLOGY, 1984, 2 :103-126

[8] GLYCOSPHINGOLIPID EXPRESSION ON MURINE L1-FIBROSARCOMA CELLS - ANALYSIS OF CLONAL INVIVO AND INVITRO SELECTED SUBLINES WITH DIFFERENT LUNG COLONIZATION POTENTIAL [J].

HANISCH, FG ;

SOLTER, J ;

JANSEN, V ;

LOCHNER, A ;

PETERKATALINIC, J ;

UHLENBRUCK, G .

BRITISH JOURNAL OF CANCER, 1990, 61 (06) :813-820

[9]

IRIMURA T, 1991, CANCER RES, V51, P387

[10] UBER DAS VORKOMMEN VON NEURAMINSAURE IN DEN MUCINEN VON SCHWEINE-SUBMAXILLARIS UND PFERDE-SUBMAXILLARIS, SOWIE VON KUH-COLOSTRUM [J].

KLENK, E ;

UHLENBRUCK, G .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1956, 305 (4-6) :224-231

← 1 2 3 →