ASSESSMENT OF THE VIABILITY AND PREGNANCY POTENTIAL OF MOUSE EMBRYOS BIOPSIED AT DIFFERENT PREIMPLANTATION STAGES OF DEVELOPMENT

被引：46
作者：
KRZYMINSKA, UB [1 ]
LUTJEN, J [1 ]
ONEILL, C [1 ]
机构：
[1] ROYAL N SHORE HOSP,HUMAN REPROD UNIT,ST LEONARDS,NSW 2065,AUSTRALIA
来源：
HUMAN REPRODUCTION | 1990年 / 5卷 / 02期
关键词：
Biopsy; Embryo; Embryo-viability; Micromanipula-tion; Prenatal diagnosis;
D O I：
10.1093/oxfordjournals.humrep.a137070
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
The developmental potential in vitro and in vivo of preimplantation mouse embryos biopsied at the 4-cell, 8-cell and morula stages were investigated. Biopsy had the least impact when performed at the 8-cell stage. There was no effect of biopsy on the development of 8-cells of blastocysts in vitro (95% compared with 99% of controls) or the implantation rate after transfers (82 versus 87%, P > 0.05); however, fewer embryos (52 versus 71%,P < 0.05) resulted in viable fetuses. There was no effect of biopsy at the 8-cell stage on fetal weight on day 17. Blastocyst formation in vitro was significantly less for 4-cell biopsies compared with their controls (76 versus 90%, P < 0.001) and biopsy also affected the implantation rate (44 versus 59%, P < 0.01). Biopsy was most detrimental when performed on morulae, reducing the implantation rate from 65% for controls to 21% for biopsies (P < 0.001). Fetal viability was also markedly affected with a reduction on day 17 from 42 to 26% accompanied by a significant reduction (24%, P = 0.02) of the mean fetal weight. Handling of embryos for biopsy at the morula stage, which involved removal of the zona pellucida, was a significant but not complete cause of the reduced implantation potential observed (sham-controls and intact-controls: 34 and 65%, P < 0.001), while puncture of the zona during the biopsy of 4-cell and 8-cell embryos had no effect. Therefore, the 8-cell mouse embryo is the most suitable state for embryo biopsy. © 1990 Oxford University Press.
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页码：203 / 208
页数：6
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