BIOGENESIS OF CHROMOMYCIN-A3 BY STREPTOMYCES-GRISEUS

The biosynthesis of chromomycin A3 was investigated using 13C-labeled acetates, methionine and glucose, and 13C, 18O-labeled acetate. 13C NMR spectral analysis demonstrated that: Aglycone assembly occurs by combining at least two polyketide chains; three of nine oxygen atoms of the aglycone originate from acetate precursor oxygen atoms; carbon methylations on the aromatic ring at C-7, on the chromose B sugar, and two O-methylations appear to be carried out by S-adenosyl-methionine requiring methyl transferases; and glucose is the precursor for all of the sugars. © 1990, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.