BIOSYNTHETIC INCORPORATION OF NONNATURAL AMINO-ACIDS INTO PROTEINS

被引：13
作者：
BRUNNER, J
机构：
[1] Department of Biochemistry, ETH-Zentrum, CH-8092 Zürich, Switzerland
[2] HTL Burgdorf
[3] ETH Zürich
[4] ETH, Department of Biochemistry
来源：
CHEMICAL SOCIETY REVIEWS | 1993年 / 22卷 / 03期
关键词：
D O I：
10.1039/cs9932200183
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This article summarizes recently developed methods of incorporating non-natural amino acids into proteins. These methods rely on the chemical aminoacylation of artificial [(semi)synthetic or in vitro transcribed] transfer RNAs that recognize a unique 'blank' codon in appropriately engineered messenger RNAs. By introducing 'designer' amino acids, it is possible to generate novel probes of protein structure and function.
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页码：183 / 189
页数：7
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