INACTIVATION OF AUXIN IN TOBACCO TRANSFORMED WITH THE INDOLEACETIC-ACID LYSINE SYNTHETASE GENE OF PSEUDOMONAS-SAVASTANOI

被引:186
作者
ROMANO, CP
HEIN, MB
KLEE, HJ
机构
[1] MONSANTO CO, ST LOUIS, MO 63198 USA
[2] Scripps Res Inst, RES INST, LA JOLLA, CA 92037 USA
关键词
AUXIN; IAA CONJUGATES; PLANT DEVELOPMENT;
D O I
10.1101/gad.5.3.438
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The iaaL gene of Pseudomonas syringae, subspecies savastanoi, encodes an indoleacetic acid (IAA)-lysine synthetase. To determine the effects of converting IAA to IAA-lysine in whole plants, the iaaL gene was fused to a constitutive plant promoter and introduced into tobacco plants. Biochemical analyses show that endogenous IAA is reduced by up to 19-fold in iaaL plants. Tobacco plants expressing the iaaL gene display reduced apical dominance, reduced rooting, and inhibition of vascular differentiation. The phenotypic effects of iaaL gene expression are reverted by crossing iaaL plants with plants that overproduce IAA. These data indicate that iaaL can act as an anti-auxin gene in vivo and confirm the role of auxin in the control of apical dominance, root growth, and vascular differentiation in whole plants.
引用
收藏
页码:438 / 446
页数:9
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