MOLECULAR-CLONING, SEQUENCE-ANALYSIS, AND EXPRESSION OF THE YEAST ALCOHOL ACETYLTRANSFERASE GENE

被引：176

作者：

FUJII, T ^{[1
]}

NAGASAWA, N ^{[1
]}

IWAMATSU, A ^{[1
]}

BOGAKI, T ^{[1
]}

TAMAI, W ^{[1
]}

HAMACHI, M ^{[1
]}

机构：

[1] OZEKI CORP, GEN RES LAB, NISHINOMIYA, HYOGO 663, JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 08期

关键词：

D O I：

10.1128/AEM.60.8.2786-2792.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ATF1 gene, which encodes alcohol acetyltransferase (AATase), was cloned from Saccharomyces cerevisiae and brewery lager yeast (Saccharomyces uvarum). The nucleotide sequence of the ATF1 gene isolated from S. cerevisiae was determined. The structural gene consists of a 1,575-bp open reading frame that encodes 525 amino acids with a calculated molecular weight of 61,059. Although the yeast AATase is considered a membrane-bound enzyme, the results of a hydrophobicity analysis suggested that this gene product does not have a membrane-spanning region that is significantly hydrophobic. A Southern analysis of the yeast genomes in which the ATF1 gene was used as a probe revealed that S. cerevisiae has one ATF1 gene, while brewery lager yeast has one ATF1 gene and another, homologous gene (Lg-ATFI). Transformants carrying multiple copies of the ATF1 gene or the Lg-ATF1 gene exhibited high AATase activity in static cultures and produced greater concentrations of acetate esters than the control.

引用

页码：2786 / 2792

页数：7

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