DETERMINATION OF ORGANIC HYDROPEROXIDE USING INDAMINE DYE FORMATION CATALYZED BY WATER-SOLUBLE IRON-PORPHYRIN AND ITS REACTION-MECHANISM

被引：4
作者：
NAKANO, T
SAKIDA, M
MIYATA, S
HONDA, H
机构：
[1] Department of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama 930-01, Sugitani
来源：
ANALYTICAL SCIENCES | 1993年 / 9卷 / 04期
关键词：
HYDROPEROXIDE; LIPID PEROXIDE; HEMIN; PORPHYRIN; INDAMINE DYE; SPECTROSCOPIC DETERMINATION;
D O I：
10.2116/analsci.9.459
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An assay method for determining organic hydroperoxides based on indamine dye formation using a water-soluble iron(III) porphyrin as catalyst was developed. The assay was performed at pH 4.4 under anaerobic conditions and was applicable to flow-injection analysis. t-Butyl hydroperoxide was determined over the range of 1 to 40 nmol with r=0.999 and SD(%)=2.4(n=5 at 4.8 nmol). The mechanism of the catalytic reaction is discussed based on kinetic measurements, suggesting a general acid-base catalyzed reaction involving a heterolytic cleavage of the O-O bond.
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页码：459 / 465
页数：7
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