BLASTOGENIC TEST FOR FOOT-AND-MOUTH-DISEASE

被引:9
作者
WARDLEY, RC
CHAPMAN, WG
GARLAND, AJM
机构
[1] Animal Virus Research Institute, Pirbright, Surrey
关键词
D O I
10.1017/S0022172400026358
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 × 105 cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2–3 days. © 1979, Cambridge University Press
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页码:507 / 512
页数:6
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