CHARACTERIZATION OF 5'-AMP-ACTIVATED PROTEIN-KINASE IN HUMAN LIVER USING SPECIFIC PEPTIDE-SUBSTRATES AND THE EFFECTS OF 5'-AMP ANALOGS ON ENZYME-ACTIVITY

被引：77

作者：

SULLIVAN, JE ^{[1
]}

CAREY, F ^{[1
]}

CARLING, D ^{[1
]}

BERI, RK ^{[1
]}

机构：

[1] ROYAL POSTGRAD MED SCH,MRC,MOLEC MED GRP,LONDON W12 0NN,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.1627

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific peptide (SAMS peptide) phosphorylation assay has previously been used to measure and subsequently purify rat liver 5'-AMP-activated protein kinase (AMPK). In this report, we show that this peptide and a peptide based on the sequence surrounding the site phosphorylated on 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase by AMPK (HMG peptide) can be used to measure human liver AMPK. Our data demonstrate that both human and rat AMPKs have a higher affinity for the HMG peptide compared to the SAMS peptide. We have used these peptide phosphorylation assays to identify novel activators of AMPK. (C) 1994 Academic Press, Inc.

引用

页码：1551 / 1556

页数：6

共 18 条

[1]

BEG ZH, 1987, J BIOL CHEM, V262, P13228

[2]

BEG ZH, 1985, J BIOL CHEM, V260, P1682

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] A COMMON BICYCLIC PROTEIN-KINASE CASCADE INACTIVATES THE REGULATORY ENZYMES OF FATTY-ACID AND CHOLESTEROL-BIOSYNTHESIS [J].

CARLING, D ;

ZAMMIT, VA ;

HARDIE, DG .

FEBS LETTERS, 1987, 223 (02) :217-222

[5] PURIFICATION AND CHARACTERIZATION OF THE AMP-ACTIVATED PROTEIN-KINASE - COPURIFICATION OF ACETYL-COA CARBOXYLASE KINASE AND 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE KINASE-ACTIVITIES [J].

CARLING, D ;

CLARKE, PR ;

ZAMMIT, VA ;

HARDIE, DG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 186 (1-2) :129-136

[6] REGULATION OF HMG-COA REDUCTASE - IDENTIFICATION OF THE SITE PHOSPHORYLATED BY THE AMP-ACTIVATED PROTEIN-KINASE INVITRO AND IN INTACT RAT-LIVER [J].

CLARKE, PR ;

HARDIE, DG .

EMBO JOURNAL, 1990, 9 (08) :2439-2446

[7] TISSUE DISTRIBUTION OF THE AMP-ACTIVATED PROTEIN-KINASE, AND LACK OF ACTIVATION BY CYCLIC-AMP-DEPENDENT PROTEIN-KINASE, STUDIED USING A SPECIFIC AND SENSITIVE PEPTIDE ASSAY [J].

DAVIES, SP ;

CARLING, D ;

HARDIE, DG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 186 (1-2) :123-128

[8] IDENTIFICATION AND ROLE OF THE BASAL PHOSPHORYLATION SITE ON HORMONE-SENSITIVE LIPASE [J].

GARTON, AJ ;

YEAMAN, SJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 191 (01) :245-250

[9] PHOSPHORYLATION OF BOVINE HORMONE-SENSITIVE LIPASE BY THE AMP-ACTIVATED PROTEIN-KINASE - A POSSIBLE ANTILIPOLYTIC MECHANISM [J].

GARTON, AJ ;

CAMPBELL, DG ;

CARLING, D ;

HARDIE, DG ;

COLBRAN, RJ ;

YEAMAN, SJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 179 (01) :249-254

[10] PHOSPHORYLATION AND INACTIVATION OF HMG-COA REDUCTASE AT THE AMP-ACTIVATED PROTEIN-KINASE SITE IN RESPONSE TO FRUCTOSE TREATMENT OF ISOLATED RAT HEPATOCYTES [J].

GILLESPIE, JG ;

HARDIE, DG .

FEBS LETTERS, 1992, 306 (01) :59-62

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