CISPLATIN BLOCKS MECHANOELECTRIC TRANSDUCER CURRENT IN CHICK COCHLEAR HAIR-CELLS

被引：32
作者：
KIMITSUKI, T
NAKAGAWA, T
HISASHI, K
KOMUNE, S
KOMIYAMA, S
机构：
[1] Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, 812
来源：
HEARING RESEARCH | 1993年 / 71卷 / 1-2期
关键词：
HAIR CELL; CISPLATIN; MECHANOELECTRIC TRANSDUCTION CHANNEL; PATCH CLAMP TECHNIQUE; OTOTOXICITY;
D O I：
10.1016/0378-5955(93)90021-R
中图分类号：
R36 [病理学]; R76 [耳鼻咽喉科学];
学科分类号：
100104 ; 100213 ;
摘要：
The effects of cisplatin (cis-dichlorodiammine platinum II, CDDP) on the mechano-electrical transduction (MET) current were investigated with a whole-cell patch-electrode voltage clamp technique in dissociated cochlear hair cells of chicks. CDDP blocked the MET channel in a dose-and voltage-dependent manner. At -50 mV, CDDP blocked the MET channel with a Hill coefficient of approximately 2 and a dissociation constant (K-D) of 1.5 x10(-3) M. The kinetics of CDDP blockade consist of a voltage-independent and a voltage-dependent component.
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页码：64 / 68
页数：5
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