IDENTIFICATION OF ACTIN-FILAMENTS IN THE RHABDOMERAL MICROVILLI OF DROSOPHILA PHOTORECEPTORS

被引:106
作者
ARIKAWA, K
HICKS, JL
WILLIAMS, DS
机构
[1] YOKOHAMA CITY UNIV,DEPT BIOL,KANAZAWA KU,YOKOHAMA 236,JAPAN
[2] INDIANA UNIV,INST MOLEC & CELLULAR BIOL,BLOOMINGTON,IN 47405
关键词
D O I
10.1083/jcb.110.6.1993
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The phototransductive microvilli of arthropod photoreceptors each contain an axial cytoskeleton. The present study shows that actin filaments are a component of this cytoskeleton in Drosophila. Firstly, actin was detected in the rhabdomeral microvilli and in the subrhabdomeral cytoplasm by immunogold labeling with antiactin. Secondly, the rhabdomeres were labeled with phalloidin, indicating the presence of filamentous actin. Finally, the actin filaments were decorated with myosin subfragment-1. The characteristic arrowhead complex formed by subfragment-1 decoration points towards the base of the microvilli, so that the fast growing end of each filament is at the distal end of the microvillus, where it is embedded in a detergent-resistant cap. Each microvillus contains more than one actin filament. Decorated filaments extend the entire length of each microvillus and project into the subrhabdomeral cytoplasm. This organization is comparable to that of the actin filaments in intestinal brush border microvilli. Similar observations were made with the photoreceptor microvilli of the crayfish, Procambarus. Our results provide an indication as to how any myosin that is associated with the rhabdomeres might function.
引用
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页码:1993 / 1998
页数:6
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