AN ENZYMATIC ASSAY OF 2,3-DIPHOSPHOGLYCERATE IN BLOOD

被引：77
作者：
NYGAARD, SF
RORTH, M
机构：
[1] Department of Clinical, Chemistry A, Rigshospitalet, Copenhagen
来源：
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1969年 / 24卷 / 04期
关键词：
2,3-diphosphoglycerate; Erythrocytes;
D O I：
10.3109/00365516909080179
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
2,3-diphosphoglycerate was estimated by an enzymatic method, based on the catalytical effect of 2,3-diphosphoglycerate on the phosphoglycerate mutase reaction, which was coupled with that of lactate dehydrogenase by adding ADP, enolase, pyruvate kinase, and NADH to the system. The activity was estimated from the decrease in NADH-concentration. Samples were obtained by diluting whole blood 100 times with oxygenated water, without any other pretreatment of the blood. The normal range of 2,3-diphosphoglycerate concentration in blood from normal individuals was determined. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码：399 / &
相关论文
共 8 条
[1]  
ASTRUP P, 1968, SCAND J CLIN LAB INV, V22, P171
[2]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P469
[3]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P459
[4]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[5]   INTRACELLULAR ORGANIC PHOSPHATES AS REGULATORS OF OXYGEN RELEASE BY HAEMOGLOBIN [J].
BENESCH, R ;
BENESCH, RE .
NATURE, 1969, 221 (5181) :618-&
[6]  
CARTIER P, 1967, ANN BIOL CLIN-PARIS, V25, P1119
[7]  
HJELM M, 1969, SCAND J HAEMATOL, V6, P56
[8]  
TOWNE JC, 1957, J BIOL CHEM, V226, P777
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