DISTRIBUTION OF DISTINCT ARACHIDONOYL-SPECIFIC AND NONSPECIFIC ISOENZYMES OF DIACYLGLYCEROL KINASE IN BABOON (PAPIO-CYNOCEPHALUS) TISSUES

被引：45

作者：

LEMAITRE, RN ^{[1
]}

KING, WC ^{[1
]}

MACDONALD, ML ^{[1
]}

GLOMSET, JA ^{[1
]}

机构：

[1] UNIV WASHINGTON,REG PRIMATE CTR,SEATTLE,WA 98195

来源：

BIOCHEMICAL JOURNAL | 1990年 / 266卷 / 01期

关键词：

D O I：

10.1042/bj2660291

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the diacylglycerol kinase species present in several baboon tissues using the substrates sn-1-stearoyl-2-arachidonoyl diacylglycerol and sn-1,2-didecanoyl diacylglycerol. Chromatography of octyl glucoside extracts of the baboon (Papio cynocephalus papio) tissues on hydroxyapatite columns revealed the presence of three diacylglycerol kinase species with different substrate preferences. One species markedly 'preferred' the substrate sn-1-stearoyl-2-arachidonoylglycerol, the two other species preferred sn-1,2-didecanoylglycerol. Measurement of the activity of the baboon brain diacylglycerol kinases toward diacylglycerols with a range of different fatty acid chains revealed a strict preference of the arachidonoyl diacylglycerol kinase for sn-1-acyl-2-arachidonoyl diacylglycerol, whereas the other enzymes showed no preference toward several long-chain-fatty-acid-containing diacylglycerols. The arachidonoyl diacylglycerol kinase was particularly abundant in brain and testis, whereas liver was practically devoid of this enzyme. The arachidonoyl diacylglycerol kinase from baboon brain was found to be predominantly associated with the particulate fraction and exhibited an apparent molecular mass of 130 kDa.

引用

页码：291 / 299

页数：9

共 26 条

[1]

BERRIDGE MJ, 1987, ANNU REV BIOCHEM, V56, P159, DOI 10.1146/annurev.bi.56.070187.001111

[2] DIACYLGLYCEROL-INDUCED TRANSLOCATION OF DIACYLGLYCEROL KINASE - USE OF AFFINITY-PURIFIED ENZYME IN A RECONSTITUTION SYSTEM [J].