RAPID MAGNETIC PURIFICATION OF ROSETTE-FORMING LYMPHOCYTES

被引：0

作者：

OWEN, CS ^{[1
]}

WINGER, LA ^{[1
]}

SYMINGTON, FW ^{[1
]}

NOWELL, PC ^{[1
]}

机构：

[1] UNIV PENN, SCH MED, DEPT BIOCHEM BIOPHYS, PHILADELPHIA, PA 19104 USA

来源：

JOURNAL OF IMMUNOLOGY | 1979年 / 123卷 / 04期

关键词：

D O I：

暂无

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

High gradient magnetic separation, which was previously shown effective in extracting erythrocytes from a flowing cell suspension was used to separate rosetted and unrosetted human peripheral blood lymphocytes. The Hb in the sheep red cells used to form rosettes was first oxidized to the paramagnetic methemoglobin form. Samples of 50 .times. 106 lymphocytes could be processed in 10 min under sterile conditions with greater than 90% purity of the rosetted cell fraction and maintenance of T [thymus-derived] cell function in mixed lymphocyte cultures.

引用

页码：1778 / 1780

页数：3

共 10 条

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