A COMMON FUNCTION FOR MESSENGER-RNA-5' AND MESSENGER-RNA-3' ENDS IN TRANSLATION INITIATION IN YEAST

被引：335

作者：

TARUN, SZ ^{[1
]}

SACHS, AB ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720

来源：

GENES & DEVELOPMENT | 1995年 / 9卷 / 23期

关键词：

TRANSLATION INITIATION; MESSENGER-RNA; RIBOSOME; YEAST; POLY(A);

D O I：

10.1101/gad.9.23.2997

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The mRNA poly(A) tail and its associated poly(A) binding protein (Pab1p) are ubiquitous in eukaryotes. The function of the poly(A) tail is to stabilize mRNA and to stimulate its translation The development of a poly(A)- and cap-dependent yeast in vitro translation system has allowed us to understand how poly(A) stimulates translation. We find that Pab1p but not the cap binding protein eIF-4E is required for poly(A) tail dependent translation, and that the Pab1p-poly(A) tail complex functions to recruit the 40S ribosomal subunit to the mRNA. These data introduce a new step into the pathway of translation initiation and merge the translational functions of the two ends of mRNA.

引用

页码：2997 / 3007

页数：11

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