STUDIES ON BIOGENIC-AMINES AND THEIR METABOLITES IN NERVOUS-TISSUE AND HEMOLYMPH OF MALE CABBAGE-LOOPER MOTHS .2. PHOTOPERIOD CHANGES RELATIVE TO RANDOM LOCOMOTOR-ACTIVITY AND PHEROMONE-RESPONSE THRESHOLDS

被引:18
作者
LINN, CE
CAMPBELL, MG
POOLE, KR
ROELOFS, WL
机构
[1] Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 1994年 / 108卷 / 01期
基金
美国国家科学基金会;
关键词
BIOGENIC AMINES; METABOLITES; OCTOPAMINE; 5-HYDROXYTRYPTAMINE; DOPAMINE; TRICHOPLUSIA NI; SCOTOPHASE;
D O I
10.1016/1367-8280(94)90093-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Levels of octopamine, 5-hydroxytryptamine, dopamine and their metabolites were measured in brain, corpora cardiaca/corpora allata complex, thoracic ganglia and optic lobes from individual male cabbage looper moths, Trichoplusia ni, using HPLC with electrochemical detection. Measurements were made from individuals in late photophase and compared with levels from individuals at mid-scotophase. Individuals at mid-scotophase had been observed for locomotor activity throughout the dark period and then assayed in the sustained-flight tunnel with a low, threshold, dose of sex pheromone prior to dissection. Individuals were selected for dissection if they exhibited a complete upwind flight to the pheromone source, or if they did not respond at all. Mid-scotophase levels of octopamine in the brain and thoracic ganglia, dopamine in the thoracic ganglia, and 5-hydroxytryptamine in the brain and thoracic ganglia were all significantly lower than photophase levels. Scotophase levels of octopamine in individuals that responded to a low dose of pheromone were significantly lower than levels from males that did not respond. Levels of random locomotor activity over the scotophase were not correlated with the probability of a male responding to the threshold dose of pheromone. There also was no correlation between brain mid-scotophase levels of amines and the degree of locomotor activity exhibited, but there was a strong correlation between decreased levels of octopamine and dopamine in the thoracic ganglia and high levels of locomotor activity. Decreased levels of octopamine in the brain and thoracic ganglia over the dark period were correlated with increased levels of N-acetyloctopamine, suggesting that utilization of the amines occurs within the tissues over this time period. Mid-scotophase levels of N-acetyldopamine were significantly greater than photophase levels, suggesting increased metabolism of this amine as well. Our results indicate that changes in amine levels within specific tissues can be correlated with behaviors exhibited by individual insects. The results support the hypothesis that octopamine, dopamine and 5-hydroxytryptamine exert modulatory or transmitter roles in the central nervous system of the cabbage looper moth.
引用
收藏
页码:87 / 98
页数:12
相关论文
共 63 条
  • [1] CLONING, LOCALIZATION, AND PERMANENT EXPRESSION OF A DROSOPHILA OCTOPAMINE RECEPTOR
    ARAKAWA, S
    GOCAYNE, JD
    MCCOMBIE, WR
    URQUHART, DA
    HALL, LM
    FRASER, CM
    VENTER, JC
    [J]. NEURON, 1990, 4 (03) : 343 - 354
  • [2] ARBAS E A, 1990, Society for Neuroscience Abstracts, V16, P758
  • [3] BAKER TC, 1985, COMPREHENSIVE INSECT, V9, P621
  • [4] EFFERENT OPTIC-NERVE FIBERS MEDIATE CIRCADIAN-RHYTHMS IN LIMULUS EYE
    BARLOW, RB
    BOLANOWSKI, SJ
    BRACHMAN, ML
    [J]. SCIENCE, 1977, 197 (4298) : 86 - 89
  • [5] BARLOW RB, 1979, INVEST OPHTH VIS SCI, P108
  • [6] A QUANTITATIVE BIOASSAY FOR SEX PHEROMONE OF EPIPHYAS POSTVITTANA (LEPIDOPTERA) AND FACTORS LIMITING MALE RESPONSIVENESS
    BARTELL, RJ
    SHOREY, HH
    [J]. JOURNAL OF INSECT PHYSIOLOGY, 1969, 15 (01) : 33 - &
  • [7] SEROTONIN IMMUNOREACTIVE NEURONS IN THE CENTRAL NERVOUS-SYSTEM OF AN INSECT (PERIPLANETA-AMERICANA)
    BISHOP, CA
    OSHEA, M
    [J]. JOURNAL OF NEUROBIOLOGY, 1983, 14 (04): : 251 - 269
  • [8] IDENTIFICATION OF NEW SEX-PHEROMONE COMPONENTS IN TRICHOPLUSIA-NI, PREDICTED FROM BIOSYNTHETIC PRECURSORS
    BJOSTAD, LB
    LINN, CE
    DU, JW
    ROELOFS, WL
    [J]. JOURNAL OF CHEMICAL ECOLOGY, 1984, 10 (09) : 1309 - 1323
  • [9] CARDE RT, 1985, S R ENTOMOL SOC LOND, V12, P241
  • [10] MALE-SPECIFIC, SEX PHEROMONE-SELECTIVE PROJECTION NEURONS IN THE ANTENNAL LOBES OF THE MOTH MANDUCASEXTA
    CHRISTENSEN, TA
    HILDEBRAND, JG
    [J]. JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 1987, 160 (05) : 553 - 569