TISSUE, DEVELOPMENTAL, AND TUMOR-SPECIFIC EXPRESSION OF DIVERGENT TRANSCRIPTS IN WILMS-TUMOR

被引：160

作者：

HUANG, A

CAMPBELL, CE

BONETTA, L

MCANDREWSHILL, MS

CHILTONMACNEILL, S

COPPES, MJ

LAW, DJ

FEINBERG, AP

YEGER, H

WILLIAMS, BRG

机构：

[1] HOSP SICK CHILDREN,RES INST,DEPT GENET,TORONTO M5G 1X8,ONTARIO,CANADA

[2] UNIV TORONTO,DEPT MOLEC & MED GENET,TORONTO M5S 1A8,ONTARIO,CANADA

[3] HOSP SICK CHILDREN,DIV HEMATOL ONCOL,TORONTO M5G 1X8,ONTARIO,CANADA

[4] HOSP SICK CHILDREN,DEPT PATHOL,TORONTO M5G 1X8,ONTARIO,CANADA

[5] UNIV TORONTO,DEPT MICROBIOL,TORONTO M5S 1A8,ONTARIO,CANADA

[6] UNIV MICHIGAN,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109

[7] UNIV MICHIGAN,DEPT INTERNAL MED,ANN ARBOR,MI 48109

[8] UNIV MICHIGAN,DEPT HUMAN GENET,ANN ARBOR,MI 48109

来源：

SCIENCE | 1990年 / 250卷 / 4983期

关键词：

D O I：

10.1126/science.2173145

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Wilms tumor locus on chromosome 11p13 has been mapped to a region defined by overlapping, tumor-specific deletions. Complementary DNA clones representing transcripts of 2.5 (WIT-1) and 3.5 kb (WIT-2 mapping to this region were isolated from a kidney complementary DNA library. Expression of WIT-1 and WIT-2 was restricted to kidney and spleen. RNase protection revealed divergent transcription of WIT-1 and WIT-2, originating from a DNA region of <600 bp. Both transcripts were present at high concentrations in fetal kidney and at much reduced amounts in 5-year-old and adult kidneys. Eleven of 12 Wilms tumors classified as histopathologically heterogeneous exhibited absent or reduced expression of WIT-2, whereas only 4 of 14 histopathologically homogeneous tumors showed reduced expression. These data demonstrate a molecular basis for the pathogenetic heterogeneity in Wilms tumorigenesis.

引用

页码：991 / 994

页数：4

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