PURIFICATION AND CHARACTERIZATION OF A CELL-WALL HYDROLASE ENCODED BY THE CW1A GENE OF BACILLUS-SUBTILIS

被引：0

作者：

KURODA, A ^{[1
]}

IMAZEKI, M ^{[1
]}

SEKIGUCHI, J ^{[1
]}

机构：

[1] SHINSHU UNIV,FAC TEXT SCI & TECHNOL,DEPT APPL BIOL,3-15-1 TOKIDA,MATSUMOTO,NAGANO 390,JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 81卷 / 01期

关键词：

CELL WALL HYDROLASE; PEPTIDOGLYCAN; BACILLUS-SUBTILIS; L-ALANINE AMIDASE;

D O I：

10.1016/0378-1097(91)90462-J

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cell wall hydrolase of Bacillus subtilis was prepared from Escherichia coli cells harboring a plasmid containing the B. subtilis cw1A gene and purified by hydroxyapatite column chromatography and HPLC through TSK-gel G3000SW(XL). In contrast to the molecular mass of 29 919 Da deduced from its nucleotide sequence, the purified CWLA is a 23 kDa protein. Characterization of the specific substrate bond cleaved by CWLA indicated that enzyme is an N-acetylmuramyl-L-alanine amidase. A 32-kDa precursor protein was detected on zymography of a crude cell homogenate. Some of the enzymatic properties of CWLA are also described.

引用

页码：9 / 14

页数：6

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