PURIFICATION AND CHARACTERIZATION OF A NEUTRAL ENDOXYLANASE FROM ASPERGILLUS-NIDULANS

被引：0

作者：

FERNANDEZESPINAR, MT

PINAGA, F

SANZ, P

RAMON, D

VALLES, S

机构：

[1] CSIC, INST AGROQUIM & TECNOL ALIMENTOS, UNIDAD BIOINGN, JAIME ROIG 11, E-46010 VALENCIA, SPAIN

[2] CSIC, INST AGROQUIM & TECNOL, UNIDAD CEREALES, E-46010 VALENCIA, SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 113卷 / 02期

关键词：

ASPERGILLUS-NIDULANS; XYLANASE; PURIFICATION;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A neutral endoxylanase from a culture filtrate of Aspergillus nidulans grown on oat spelt xylan was purified to apparent homogeneity. The purified enzyme showed a single band on SDS-PAGE with a molecular mass of 22,000 and had an isoelectric point of 6.4. The enzyme was a non-debranching endoxylanase highly specific for xylans and completely free from cellulolytic activity. The xylanase showed an optimum activity at pH 5.5 and 62-degrees-C and had a K(m) of 4.2 mg oat spelt xylan per ml and a V(max) of 710 mumol min-1 (mg protein)-1.

引用

页码：223 / 228

页数：6

共 14 条

[1] MICROBIAL XYLANOLYTIC SYSTEMS [J].