INVITRO DEGRADATION OF ENDOTHELIN-1 BY ENDOPEPTIDASE 24.11 (ENKEPHALINASE)

被引：39

作者：

FAGNY, C

MICHEL, A

LEONARD, I

BERKENBOOM, G

FONTAINE, J

DESCHODTLANCKMAN, M

机构：

[1] UNIV LIBRE BRUXELLES,FAC MED,PLURIDISCIPLINAIRE RECH EXPTL BIOMED LAB,BATIMENT C,CAMPUS ERASME,B-1070 BRUSSELS,BELGIUM

[2] UNIV LIBRE BRUXELLES,INST PHARM,DEPT PHARMACOL,B-1050 BRUSSELS,BELGIUM

[3] UNIV MONS,FAC SCI,CHIM BIOL LAB,B-7000 MONS,BELGIUM

来源：

PEPTIDES | 1991年 / 12卷 / 04期

关键词：

ENDOPEPTIDASE; 24.11; ENKEPHALINASE; ENDOTHELIN; PHOSPHORAMIDON; ENZYMATIC DEGRADATION;

D O I：

10.1016/0196-9781(91)90132-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The breakdown of endothelin-1 by crude membrane preparations of human kidney and choroid plexus was investigated. I-125-labeled endothelin-1 was degraded by both tissues in a phosphoramidon-sensitive way, suggesting a role of endopeptidase 24.11 in the in vitro metabolism of this peptide. Identification of the cleavage sites of purified human renal endopeptidase 24.11 in the sequence of endothelin-1 revealed that bonds involving the amino side of the hydrophobic amino acids (Ser4, Leu6, Val12, Phe14, His16, Leu17, Ile19) were susceptible to cleavage. Endothelin-1 appears thus to be degraded at multiple sites by endopeptidase 24.11 in vitro, producing inactive fragments.

引用

页码：773 / 778

页数：6

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