MOLECULAR-CLONING OF A HUMAN NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR BETA-3-LIKE SUBUNIT

被引：12
作者：
WILLOUGHBY, JJ
NINKINA, NN
BEECH, MM
LATCHMAN, DS
WOOD, JN
机构：
[1] SANDOZ INST MED RES, LONDON, ENGLAND
[2] UNIV COLL & MIDDLESEX HOSP SCH MED, DIV MOLEC PATHOL, WINDEYEN BLDG, CLEVELAND ST, LONDON W1P 6DB, ENGLAND
来源：
NEUROSCIENCE LETTERS | 1993年 / 155卷 / 02期
关键词：
NICOTINE; ACETYLCHOLINE; HUMAN; NEURON; ION CHANNEL;
D O I：
10.1016/0304-3940(93)90691-D
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Three cDNA clones homologous to rat neuronal nicotinic receptors were identified in a human brain stem library by screening at low stringency with a mixture of rat alpha5, beta2 and beta4 subunit probes. Positive clones were further analysed by hybridisation at higher stringency with individual rat nicotinic receptor probes. One positive clone was found to encode a protein that exhibited characteristic features of a member of the ligand-gated ion channel class of protein subunits, and showed 89% homology with a rat beta3 neuronal nicotinic receptor subunit. Northern blots demonstrated the presence of a 1.7 kb transcript in RNA extracted from adult human Pons. This clone has therefore been designated the human beta3 neuronal nicotinic receptor subunit.
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页码：136 / 139
页数：4
相关论文
共 21 条
[1]   NUCLEOTIDE-SEQUENCE OF THE HUMAN NICOTINIC ACETYLCHOLINE-RECEPTOR BETA-2 SUBUNIT GENE [J].
ANAND, R ;
LINDSTROM, J .
NUCLEIC ACIDS RESEARCH, 1990, 18 (14) :4272-4272
[2]   HOMOLOGY AND ANALOGY IN TRANSMEMBRANE CHANNEL DESIGN - LESSONS FROM SYNAPTIC MEMBRANE-PROTEINS [J].
BETZ, H .
BIOCHEMISTRY, 1990, 29 (15) :3591-3599
[3]   NICOTINIC ACETYLCHOLINE-RECEPTOR MESSENGER-RNA IN DORSAL-ROOT GANGLION NEURONS [J].
BOYD, RT ;
JACOB, MH ;
MCEACHERN, AE ;
CARON, S ;
BERG, DK .
JOURNAL OF NEUROBIOLOGY, 1991, 22 (01) :1-14
[4]   CHEMICAL TRANSMISSION IN THE RAT INTERPEDUNCULAR NUCLEUS INVITRO [J].
BROWN, DA ;
DOCHERTY, RJ ;
HALLIWELL, JV .
JOURNAL OF PHYSIOLOGY-LONDON, 1983, 341 (AUG) :655-670
[5]   NEURONAL-TYPE ALPHA-BUNGAROTOXIN RECEPTORS AND THE ALPHA-5-NICOTINIC RECEPTOR SUBUNIT GENE ARE EXPRESSED IN NEURONAL AND NONNEURONAL HUMAN CELL-LINES [J].
CHINI, B ;
CLEMENTI, F ;
HUKOVIC, N ;
SHER, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1572-1576
[6]   THE FALL AND RISE OF NEURONAL ALPHA-BUNGAROTOXIN BINDING-PROTEINS [J].
CLARKE, PBS .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1992, 13 (11) :407-413
[7]   THE ALPHA-5-GENE PRODUCT ASSEMBLES WITH MULTIPLE ACETYLCHOLINE-RECEPTOR SUBUNITS TO FORM DISTINCTIVE RECEPTOR SUBTYPES IN BRAIN [J].
CONROY, WG ;
VERNALLIS, AB ;
BERG, DK .
NEURON, 1992, 9 (04) :679-691
[8]   PHARMACOLOGICAL AND FUNCTIONAL DIVERSITY OF NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS [J].
DENERIS, ES ;
CONNOLLY, J ;
ROGERS, SW ;
DUVOISIN, R .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1991, 12 (01) :34-40
[9]  
DENERIS ES, 1989, J BIOL CHEM, V264, P6268
[10]   MOLECULAR-CLONING OF HUMAN NEURONAL NICOTINIC RECEPTOR ALPHA-3-SUBUNIT [J].
FORNASARI, D ;
CHINI, B ;
TARRONI, P ;
CLEMENTI, F .
NEUROSCIENCE LETTERS, 1990, 111 (03) :351-356
123