ENZYME MASS-TRANSFER COEFFICIENT IN AQUEOUS 2 PHASE SYSTEM USING A PACKED EXTRACTION COLUMN

被引:10
作者
PATIL, TA [1 ]
JAFARABAD, KR [1 ]
SAWANT, SB [1 ]
JOSHI, JB [1 ]
机构
[1] UNIV BOMBAY,DEPT CHEM TECHNOL,BOMBAY 400019,INDIA
关键词
AQUEOUS 2 PHASE SYSTEM; PACKED COLUMN; MASS TRANSFER COEFFICIENT; ENZYME TRANSFER;
D O I
10.1002/cjce.5450690221
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
Fractional dispersed phase hold-up and dispersed side mass transfer coefficients for amyloglucosidase were measured in a 56 mm i.d. packed extraction column using a sodium sulphate-polyethylene glycol 4000 system. Raschig rings (3 to 13.3 mm), Berl saddles (12 mm), Pall rings (12.6 mm), glass spheres (5.2 mm) and structured wire gauze were used as packings. The effect of packing size was investigated in the case of ceramic Raschig rings. The effect of phase composition of the aqueous phase system also was studied. Correlations have been developed for fractional dispersed phase hold-up and volumetric mass transfer coefficient with packing voidage, dry surface area of packings, superficial dispersed phase velocity and the liquid phase physical properties.
引用
收藏
页码:548 / 556
页数:9
相关论文
共 32 条
[1]  
Albertsson P. -<Angstrom>., 1985, PARTITION CELL PARTI
[2]  
Brooks D. E., 1985, PARTITIONING AQUEOUS
[3]  
Calderbank P.H, 1967, CHEM ENG-NEW YORK, V45, P209
[4]  
DELL FR, 1951, T I CHEM ENG-LOND, V29, P89
[5]   CONTINUOUSLY MONITORED SPECTROPHOTOMETRIC ASSAY OF GLYCOSIDASES WITH NITROPHENYL GLYCOSIDES [J].
FORD, JR ;
NUNLEY, JA ;
LI, Y ;
CHAMBERS, RP ;
COHEN, W .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (01) :120-128
[6]  
GARNER FH, 1960, ANAL REALE SOC ESP B, V46, P479
[7]  
GAYLER R, 1957, T I CHEM ENG-LOND, V35, P273
[8]  
Gayler R., 1951, T I CHEM ENG, V29, P110
[9]  
Gayler R.L., 1953, T I CHEM ENG-LOND, V31, P57
[10]  
GEANKOPOLIS CJ, 1983, TRANSPORT PROCESSES, P395