IMPROVED SENSITIVITY IS THE MONOCYTE-MEDIATED ADCC ASSAY WITH RED-CELL TARGETS

被引：1

作者：

KIRKWOOD, J ^{[1
]}

TRUEMAN, K ^{[1
]}

FLETCHER, A ^{[1
]}

机构：

[1] NEW S WALES RED CROSS,BLOOD TRANSFUS SERV,SYDNEY,NSW 2000,AUSTRALIA

来源：

TRANSFUSION MEDICINE | 1993年 / 3卷 / 04期

关键词：

ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY; ANTI-D; MONOCYTE;

D O I：

10.1111/j.1365-3148.1993.tb00060.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Monocyte-mediated antibody-dependent cellular cytotoxicity (ADCC) assays are being increasingly employed in blood transfusion to predict the severity of haemolytic disease of the new-born (HDN). In our hands the monocyte-mediated ADCC assay resulted in very high spontaneous release of Cr-51, making the assay unreliable. The addition of bovine serum albumin (BSA) to the buffer used to wash red blood cells (RBC) was found to reduce the spontaneous release to acceptable levels, improving the sensitivity of the assay. The modified assay was used to test 15 monoclonal anti-D antibodies showing that the assay results are reproducible.

引用

页码：269 / 273

页数：5

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