ENTRAPMENT OF PLASMID DNA IN LIPOSOMES

被引：26

作者：

DIMITRIADIS, GJ

机构：

[1] National Institute for Medical Research, London NW7 1AA, The Ridgeway, Mill Hill

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 08期

关键词：

D O I：

10.1093/nar/6.8.2697

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The entrapment of plasmid DNA (pMB9) and high molecular weight DNA into large unilamellar liposomes is described. The entrapment of DNA is specific and due to encapsulation of DNA into the aqueous compartment of liposomes. The entrapped DNA, resistant to deoxyribonuclease treatment, could be reisolated from liposomes intact and, as has been shown by transformation assay, it remains biologically active. The advantages of our method and possible applications are discussed. © 1979 Information Retrieval Limited.

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页码：2697 / 2705

页数：9

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