IN VITRO INTERACTION OF 1-ANILINO 8-NAPHTALENE SULFONATE WITH EXCITABLE MEMBRANES ISOLATED FROM ELECTRIC ORGAN OF ELECTROPHORUS ELECTRICUS

被引：53
作者：
KASAI, M
CHANGEUX, JP
MONNERIE, L
机构：
[1] Département de Biologie Moléculaire, Institut Pasteur, Paris
[2] Laboratoire de Chimie Macromoléculaire, Ecole de Physique et Chimie, Paris
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 36卷 / 03期
关键词：
D O I：
10.1016/0006-291X(69)90581-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In vitro binding of 1-anilino 8-naphtalene sulfonate (ANS) to membrane fragments derived from the innervated, excitable, surface of electroplax cells is accompanied by a strong enhancement of fluorescence intensity and polarization. Effects of temperature on ANS binding indicate that a structural reorganization of the membrane fragments occurs between 30 and 50°C. Fluorescence polarization of bound ANS is insensitive to changes of solvent viscosity upon addition of sucrose. Ca++ and d-tubocurarine modifies ANS binding. © 1969.
引用
收藏
页码：420 / &
相关论文
共 12 条
[1]  
ANDERSON SR, 1969, BIOCHEMISTRY, V8, P361
[2]   LOCAL ANESTHETIC INDUCED CHANGES OF A MEMBRANE-BOUND FLUOROCHROME . A LINK BETWEEN ION UPTAKE AND MEMBRANE STRUCTURE [J].
CHANCE, B ;
AZZI, A ;
MELA, L ;
RADDA, G ;
VAINIO, H .
FEBS LETTERS, 1969, 3 (01) :10-&
[3]  
CHANGEUX JP, IN PRESS
[4]   INTERACTION OF 1-ANILINO-8-NAPHTHALENE SULPHONATE WITH ERYTHROCYTE MEMBRANES [J].
FREEDMAN, RB ;
RADDA, GK .
FEBS LETTERS, 1969, 3 (02) :150-&
[5]   PHYSICOCHEMICAL PROPERTIES OF BENCE-JONES PROTEINS IN FORM OF L-CHAIN DIMERS [J].
GALLY, JA ;
EDELMAN, GM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 94 (01) :175-+
[6]   FLUORESCENT PROBES FOR CONFORMATIONAL STATES OF PROTEINS .I. MECHANISM OF FLUORESCENCE OF 2-P-TOLUIDINYLNAPHTHALENE-6-SULFONATE A HYDROPHOBIC PROBE [J].
MCCLURE, WO ;
EDELMAN, GM .
BIOCHEMISTRY, 1966, 5 (06) :1908-&
[7]  
MONNERIE L, 1969, J CHIM PHYS PCB, V61, P504
[8]   FLUORESCENCE SPECTROSCOPY OF PROTEINS [J].
STRYER, L .
SCIENCE, 1968, 162 (3853) :526-&
[9]  
STRYER LUBERT, 1965, J MOL BIOL, V13, P482
[10]   CHANGES IN FLUORESCENCE TURBIDITY AND BIREFRINGENCE ASSOCIATED WITH NERVE EXCITATION [J].
TASAKI, I ;
WATANABE, A ;
SANDLIN, R ;
CARNAY, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 61 (03) :883-&
12