RAPID PREPARATION OF DNA FROM PHYTOPATHOGENIC MYCOPLASMA-LIKE ORGANISMS FOR POLYMERASE CHAIN-REACTION ANALYSIS

被引：6

作者：

FIRRAO, G

LOCCI, R

机构：

[1] Dipartimento di Biologia Applicata Alla Difesa Delle Piante, Università di Udine, Udine, I-33100

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 17卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb01466.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple procedure for the processing of MLO (mycoplasma-like organisms)-infected plant samples for PCR analysis is described. It is based on differential centrifugation and removal of enzyme inhibiting agents with the aid of a resin. Results of the amplifications are comparable with those achieved with more complex DNA extraction procedures. The method does not require any extraction with organic solvents or alcoholic precipitation of nucleic acids.

引用

页码：280 / 281

页数：2

共 5 条

[1] DETECTION OF DNA OF PLANT PATHOGENIC MYCOPLASMALIKE ORGANISMS BY A POLYMERASE CHAIN-REACTION THAT AMPLIFIES A SEQUENCE OF THE 16S RNA GENE
AHRENS, U
SEEMULLER, E
[J]. PHYTOPATHOLOGY, 1992, 82 (08) : 828 - 832
[2] SENSITIVE DETECTION OF MYCOPLASMALIKE ORGANISMS IN FIELD-COLLECTED AND INVITRO PROPAGATED PLANTS OF BRASSICA, HYDRANGEA AND CHRYSANTHEMUM BY POLYMERASE CHAIN-REACTION
BERTACCINI, A
DAVIS, RE
HAMMOND, RW
VIBIO, M
BELLARDI, MG
LEE, IM
[J]. ANNALS OF APPLIED BIOLOGY, 1992, 121 (03) : 593 - 599
[3] ENHANCED DETECTION OF A PLANT PATHOGENIC MYCOPLASMA-LIKE ORGANISM BY POLYMERASE CHAIN-REACTION
DENG, SJ
HIRUKI, C
[J]. PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 1990, 66 (07): : 140 - 144
[4] USE OF POLYMERASE CHAIN-REACTION TO PRODUCE OLIGONUCLEOTIDE PROBES FOR MYCOPLASMALIKE ORGANISMS
FIRRAO, G
GOBBI, E
LOCCI, R
[J]. PHYTOPATHOLOGY, 1993, 83 (06) : 602 - 607
[5] SENSITIVE DETECTION AND IDENTIFICATION OF MYCOPLASMA-LIKE ORGANISMS IN PLANTS BY POLYMERASE CHAIN-REACTIONS
SCHAFF, D
LEE, IM
DAVIS, RE
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 186 (03) : 1503 - 1509

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