SOLID-PHASE INJECTOR FOR OPEN TUBULAR COLUMN SUPERCRITICAL-FLUID CHROMATOGRAPHY

The design and use of a solid-phase injector for open tubular column supercritical fluid chromatography (SFC) are reported. For sample introduction, a liquid sample was loaded on a platinum wire. After the solvent had evaporated, the wire was inserted into, and sealed in, the injector. The chromatographic run was started by introducing the supercritical mobile phase into the injector. Absolute and relative peak area reproducibilities, as well as retention time reproducibility, of the injection method were evaluated. The relative standard deviations (% RSDs) of absolute and relative peak areas were 2.0-4.5 and 0.4-1.8%, respectively, for both 1- and 2-mul injections. Larger deviations were observed for compounds that had high vapor pressures. The % RSDs of retention times from six subsequent injections were 0.04-0.06.