USE OF SYNTHETIC PEPTIDES TO MAP SEQUENTIAL EPITOPES RECOGNIZED BY MONOCLONAL-ANTIBODIES ON THE BOVINE LEUKEMIA-VIRUS EXTERNAL GLYCOPROTEIN

被引：29
作者：
CALLEBAUT, I
BURNY, A
KRCHNAK, V
GRASMASSE, H
WATHELET, B
PORTETELLE, D
机构：
[1] RES INST FEED SUPPLEMENTS & VET DRUGS, PRAGUE, CZECHOSLOVAKIA
[2] INST PASTEUR, F-59019 LILLE, FRANCE
来源：
VIROLOGY | 1991年 / 185卷 / 01期
关键词：
D O I：
10.1016/0042-6822(91)90752-W
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Six sequential epitopes (A, B, B′, D, D′, E) were previously defined on the bovine leukemia virus (BLV) envelope glycoprotein gp51 by their reactivity with monoclonal antibodies. A panel of synthetic peptides covering almost the entire sequence of gp51 was tested in enzyme-linked immunosorbent assays in order to localize these epitopes. E was shown to be included in peptide 142-161 (MCF4), B and B′ in peptide 195-205, D and D′ in peptide 218-237 (MCF6), and A in peptide 249-268 (MCF7). These results extend and confirm previous observations suggesting that the sequential epitopes recognized by our battery of monoclonal antibodies are located in the carboxylic half of BLV gp51. © 1991.
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页码：48 / 55
页数：8
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