Magnesium incorporated chitosan based scaffolds for tissue engineering applications

被引：57

作者：

Adhikari, Udhab ^{[1
,4
]}

Rijal, Nava P. ^{[2
,4
]}

Khanal, Shalil ^{[3
,4
]}

Pai, Devdas ^{[1
,4
]}

Sankar, Jagannathan ^{[1
,4
]}

Bhattarai, Narayan ^{[2
,4
]}

机构：

[1] North Carolina A&T State Univ, Dept Mech Engn, Greensboro, NC USA

[2] North Carolina A&T State Univ, Dept Chem Biol & Bioengn, Greensboro, NC 27401 USA

[3] North Carolina A&T State Univ, Dept Energy & Environm Syst, Greensboro, NC USA

[4] North Carolina A&T State Univ, NSF ERC Revolutionizing Metall Biomat, Greensboro, NC USA

来源：

BIOACTIVE MATERIALS | 2016年 / 1卷 / 02期

基金：

美国国家科学基金会;

关键词：

Chitosan; Magnesium gluconate; Carboxymethyl chitosan; Tissue engineering; Composite scaffolds;

D O I：

10.1016/j.bioactmat.2016.11.003

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Chitosan based porous scaffolds are of great interest in biomedical applications especially in tissue engineering because of their excellent biocompatibility in vivo, controllable degradation rate and tailorable mechanical properties. This paper presents a study of the fabrication and characterization of bioactive scaffolds made of chitosan (CS), carboxymethyl chitosan (CMC) and magnesium gluconate (MgG). Scaffolds were fabricated by subsequent freezing-induced phase separation and lyophilization of polyelectrolyte complexes of CS, CMC and MgG. The scaffolds possess uniform porosity with highly interconnected pores of 50-250 mu m size range. Compressive strengths up to 400 kPa, and elastic moduli up to 5 MPa were obtained. The scaffolds were found to remain intact, retaining their original threedimensional frameworks while testing in in-vitro conditions. These scaffolds exhibited no cytotoxicity to 3T3 fibroblast and osteoblast cells. These observations demonstrate the efficacy of this new approach to preparing scaffold materials suitable for tissue engineering applications. (C) 2016 The Authors. Production and hosting by Elsevier B. V. on behalf of KeAi Communications Co., Ltd.

引用

页码：132 / 139

页数：8

共 42 条

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