DETECTION OF ANTIBODY TO SALMONELLA-ENTERITIDIS AND SALMONELLA-TYPHIMURIUM IN THE YOLK OF HENS EGGS

被引:31
作者
NICHOLAS, RAJ
ANDREWS, SJ
机构
[1] Poultry Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
关键词
D O I
10.1136/vr.128.5.98
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect IgG antibodies to Salmonella enteritidis and S typhimurium in the yolk of hens' eggs. Better discrimination and more consistent results were obtained between eggs from experimentally infected and uninfected hens by using saline-dilution of yolk rather than chloroform extraction. Threshold absorbance values were determined in three salmonella-free flocks, and on the basis of these results ELISA optical density values greater than 0.25 were considered to be positive for antibodies to salmonella. Four flocks with a history of salmonella infection were examined; three contained birds which were seropositive for S enteritidis by ELISA and from which S enteritidis was isolated, and a large proportion of eggs from these birds contained antibody to S enteritidis. Eggs from the fourth flock had no detectable antibody, although serum antibody was detected in some birds. No salmonellae were isolated from the yolks of the eggs from any of the four flocks.
引用
收藏
页码:98 / 100
页数:3
相关论文
共 9 条
[1]   SEROLOGICAL RESPONSE OF CHICKENS TO SALMONELLA-ENTERITIDIS INFECTION [J].
CHART, H ;
ROWE, B ;
BASKERVILLE, A ;
HUMPHREY, TJ .
EPIDEMIOLOGY AND INFECTION, 1990, 104 (01) :63-71
[2]  
COOPER GL, 1989, VET REC, V125, P5567
[3]  
DADRAST H, 1990, VET REC, V126, P219
[4]  
MOHAMMED HO, 1986, AVIAN DIS, V28, P877
[5]   USE OF EGG-YOLK IN SEROLOGICAL TESTS (ELISA AND HI) TO DETECT ANTIBODY TO NEWCASTLE-DISEASE, INFECTIOUS-BRONCHITIS, AND MYCOPLASMA-GALLISEPTICUM [J].
PIELA, TH ;
GULKA, CM ;
YATES, VJ ;
CHANG, PW .
AVIAN DISEASES, 1984, 28 (04) :877-883
[6]   IMMUNOGLOBULINS IN THE EGG, EMBRYO AND YOUNG CHICK [J].
ROSE, ME ;
ORLANS, E .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1981, 5 (01) :15-20
[7]   Experiences and benefits of volunteering in a community AIDS organization [J].
Crook, Joan ;
Weir, Robin ;
Willms, Dennis ;
Egdorf, Thomas .
JANAC-JOURNAL OF THE ASSOCIATION OF NURSES IN AIDS CARE, 2006, 17 (04) :39-45
[8]   LIPID-PROTEIN GLOBULES OF AVIAN EGG-YOLK - ISOLATION AND PROPERTIES OF GLOBULES STABLE IN CONCENTRATED SODIUM-CHLORIDE SOLUTION [J].
VADEHRA, DV ;
BAIN, JM ;
BURLEY, RW .
BIOCHEMICAL JOURNAL, 1977, 166 (03) :619-624
[9]   UBER DIE EXTRAKTION VON BAKTERIEN MIT PHENOL WASSER [J].
WESTPHAL, O ;
LUDERITZ, O ;
BISTER, F .
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES, 1952, 7 (03) :148-155