A SOLID-PHASE REVERSE-TRANSCRIPTASE MICROASSAY FOR THE DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS AND OTHER RETROVIRUSES IN CELL-CULTURE SUPERNATANTS

被引：25

作者：

SOMOGYI, PA

GYURIS, A

FOLDES, I

机构：

[1] National Institute of Hygiene, Microbiological Research Group, Budapest

来源：

JOURNAL OF VIROLOGICAL METHODS | 1990年 / 27卷 / 03期

关键词：

Human immunodeficiency virus; PEG; Retrovirus; Reverse transcriptase;

D O I：

10.1016/0166-0934(90)90095-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12-18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or Other retroviruses in cell culture supernatants. It was found that PEG (per se) - up to 4% concentrations (w/v) - did not inhibit reverse transcriptase activity. Optimal conditions of the assay were determined. This solid-phase technique is much faster and more convenient than the methods described previously. © 1990.

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页码：269 / 276

页数：8

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