AN EFFICIENTLY EXPRESSED 5.8S RIBOSOMAL-RNA TAG FOR IN-VIVO STUDIES OF YEAST RIBOSOMAL-RNA BIOSYNTHESIS AND FUNCTION

被引：7

作者：

ABOUELELA, S ^{[1
]}

GOOD, L ^{[1
]}

NAZAR, RN ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT MOLEC BIOL & GENET,GUELPH,ON N1G 2W1,CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1262卷 / 2-3期

基金：

加拿大自然科学与工程研究理事会;

关键词：

TARGETED MUTAGENESIS; RDNA; 5.8S RIBOSOMAL-RNA; INTERNAL TRANSCRIBED SPACER;

D O I：

10.1016/0167-4781(95)00074-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inefficient expression or detrimental markers have limited mutational analyses of eukaryotic 5.88 rRNA and the associated rDNA transcribed spacers. We have found a neutral, 4-base insertion mutation that effectively tags the 5.8S rRNA for improved studies of rRNA expression, processing and function. Cells expressing the tagged rBNA plasmid contain 50-60% mutant 5.8S rRNA, but show a normal growth rate and polysomal profile and a constant distribution of tagged 5.8S rRNA. The high level of expression also demonstrates that plasmid-associated rDNA is preferentially transcribed over chromosomal copies.

引用

页码：164 / 167

页数：4

共 13 条

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