RAPID AND SENSITIVE DETERMINATION OF COUMARIN AND 7-HYDROXYCOUMARIN AND ITS GLUCURONIDE CONJUGATE IN URINE AND PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：41
作者：
EGAN, DA
OKENNEDY, R
机构：
[1] School of Biological Sciences, Dublin City University, Glasnevin
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 582卷 / 1-2期
关键词：
D O I：
10.1016/0378-4347(92)80312-E
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A rapid and sensitive high-performance liquid chromatographic method was developed for the analysis of coumarin, 7-hydroxycoumarin and its glucuronide conjugate in urine and plasma. This method was used to monitor the urinary excretion of these compounds following a single oral dose of coumarin (100 mg). This new method gives excellent chromatographic separation and includes an internal standard. The method was validated and shown to be both accurate and precise in the range 0.5-100 mug/ml.
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页码：137 / 143
页数：7
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