IDENTIFICATION OF THE N-TERMINAL RESIDUE OF THE HEAVY-CHAIN OF BOTH NATIVE AND RECOMBINANT HUMAN HEPATOCYTE GROWTH-FACTOR

被引：71

作者：

YOSHIYAMA, Y

ARAKAKI, N

NAKA, D

TAKAHASHI, K

HIRONO, S

KONDO, J

NAKAYAMA, H

GOHDA, E

KITAMURA, N

TSUBOUCHI, H

ISHII, T

HISHIDA, T

DAIKUHARA, Y

机构：

[1] KAGOSHIMA UNIV,SCH DENT,DEPT BIOCHEM,SAKURAGAOKA 8,KAGOSHIMA 890,JAPAN

[2] MITSUBISHI KASEI CORP,RES CTR,MIDORI KU,YOKOHAMA 227,JAPAN

[3] KANSAI MED UNIV,INST LIVER RES,MORIGUCHI,OSAKA 570,JAPAN

[4] KAGOSHIMA UNIV,FAC MED,DEPT INTERNAL MED 2,KAGOSHIMA 890,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91616-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The N-terminal amino acid of the heavy chain of native (purified from human plasma) and recombinant human hepatocyte growth factor (hHGF) was determined by analyses of amino acid composition and sequence of peptide fragments derived by enzymatic cleavage, peptide mapping, and fast atom bombardment mass spectrometry. Our results indicate that the N-terminal amino acid of the heavy chain of hHGF, both native and recombinant, is pyroglutamate, derived from glutamine at the 32nd residue from the initiation methionine. © 1991.

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页码：660 / 667

页数：8

相关论文

共 16 条

[1] BIOLOGICAL AND IMMUNOLOGICAL PROPERTIES OF HUMAN HEPATOCYTE GROWTH-FACTOR FROM PLASMA OF PATIENTS WITH FULMINANT HEPATIC-FAILURE [J].

GOHDA, E ;

YAMASAKI, T ;

TSUBOUCHI, H ;

KUROBE, M ;

SAKIYAMA, O ;

AOKI, H ;

NIIDANI, N ;

SHIN, S ;

HAYASHI, K ;

HASHIMOTO, S ;

DAIKUHARA, Y ;

YAMAMOTO, I .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1053 (01) :21-26

[2] PURIFICATION AND PARTIAL CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM PLASMA OF A PATIENT WITH FULMINANT HEPATIC-FAILURE [J].

GOHDA, E ;

TSUBOUCHI, H ;

NAKAYAMA, H ;

HIRONO, S ;

SAKIYAMA, O ;

TAKAHASHI, K ;

MIYAZAKI, H ;

HASHIMOTO, S ;

DAIKUHARA, Y .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (02) :414-419

[3] IDENTITY OF A TUMOR CYTOTOXIC FACTOR FROM HUMAN FIBROBLASTS AND HEPATOCYTE GROWTH-FACTOR [J].

HIGASHIO, K ;

SHIMA, N ;

GOTO, M ;

ITAGAKI, Y ;

NAGAO, M ;

YASUDA, H ;

MORINAGA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 170 (01) :397-404

[4] MARKED INCREASE OF HGF MESSENGER-RNA IN NON-PARENCHYMAL LIVER-CELLS OF RATS TREATED WITH HEPATOTOXINS [J].

KINOSHITA, T ;

TASHIRO, K ;

NAKAMURA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) :1229-1234

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR HUMAN HEPATOCYTE GROWTH-FACTOR [J].

MIYAZAWA, K ;

TSUBOUCHI, H ;

NAKA, D ;

TAKAHASHI, K ;

OKIGAKI, M ;

ARAKAKI, N ;

NAKAYAMA, H ;

HIRONO, S ;

SAKIYAMA, O ;

TAKAHASHI, K ;

GOHDA, E ;

DAIKUHARA, Y ;

KITAMURA, N .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 163 (02) :967-973

[7]

Murakami H, 1989, Adv Biotechnol Processes, V11, P107

[8] MOLECULAR-CLONING AND EXPRESSION OF HUMAN HEPATOCYTE GROWTH-FACTOR [J].

NAKAMURA, T ;

NISHIZAWA, T ;

HAGIYA, M ;

SEKI, T ;

SHIMONISHI, M ;

SUGIMURA, A ;

TASHIRO, K ;

SHIMIZU, S .

NATURE, 1989, 342 (6248) :440-443

[9] TRANSFORMATION OF MOUSE FIBROBLASTS TO METHOTREXATE RESISTANCE BY A RECOMBINANT PLASMID EXPRESSING A PROKARYOTIC DIHYDROFOLATE-REDUCTASE [J].

OHARE, K ;

BENOIST, C ;

BREATHNACH, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (03) :1527-1531

[10] PRIMARY STRUCTURE OF RAT HEPATOCYTE GROWTH-FACTOR AND INDUCTION OF ITS MESSENGER-RNA DURING LIVER-REGENERATION FOLLOWING HEPATIC-INJURY [J].

OKAJIMA, A ;

MIYAZAWA, K ;

KITAMURA, N .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 193 (02) :375-381