ENDONUCLEASE CLEAVAGE MAP OF THE PLASMID PWWO-8, A DERIVATIVE OF THE TOL PLASMID OF PSEUDOMONAS-PUTIDA MT-2

被引：30

作者：

DOWNING, RG ^{[1
]}

DUGGLEBY, CJ ^{[1
]}

VILLEMS, R ^{[1
]}

BRODA, P ^{[1
]}

机构：

[1] UNIV EDINBURGH,DEPT MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 168卷 / 01期

关键词：

D O I：

10.1007/BF00267938

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cleavage sites on the pWWO-8 plasmid were determined for the restriction endonucleases HindIII and XhoI. Terminal labelling using DNA polymerase I was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasmid. © 1979 Springer-Verlag.

引用

页码：97 / 99

页数：3

共 5 条

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[4] NUCLEOTIDE SEQUENCE ANALYSIS WITH POLYNUCLEOTIDE KINASE AND NUCLEOTIDE MAPPING METHODS - 5'-TERMINAL SEQUENCES OF DEOXYRIBONUCLEIC ACID FROM BACTERIOPHAGES LAMBDA AND 424 [J].

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BIOCHEMICAL JOURNAL, 1973, 131 (03) :569-&

[5] RESTRICTION ENDONUCLEASE MAPPING OF DNA USING INSITU DIGESTION IN 2-DIMENSIONAL GELS [J].

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