THE N-TERMINAL PART OF THE ESCHERICHIA-COLI DNA-BINDING PROTEIN FIS IS ESSENTIAL FOR STIMULATING SITE-SPECIFIC DNA INVERSION BUT IS NOT REQUIRED FOR SPECIFIC DNA-BINDING

被引：61

作者：

KOCH, C ^{[1
]}

NINNEMANN, O ^{[1
]}

FUSS, H ^{[1
]}

KAHMANN, R ^{[1
]}

机构：

[1] INST GENBIOL FORSCH BERLIN GMBH,IHNESTR 63,W-1000 BERLIN 33,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 21期

关键词：

D O I：

10.1093/nar/19.21.5915

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

FIS protein is involved in several different cellular processes stimulating site-specific recombination in phages Mu and lambda as well as transcription of stable RNA operons in E. coli. We have performed a mutational analysis of fis and provide genetic and biochemical evidence that a truncated version of FIS lacking the N-terminal region is sufficient for specific DNA binding and for stimulating lambda-excision. These mutants also retain their ability to autoregulate fis gene expression. Such mutant proteins, however, cannot stimulate the enhancer dependent DNA inversion reaction.

引用

页码：5915 / 5922

页数：8

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