DECREASE OF MELTING TEMPERATURE AND SINGLE STRAND SCISSION OF DNA BY BLEOMYCIN IN PRESENCE OF 2-MERCAPTOETHANOL

被引：106

作者：

NAGAI, K

SUZUKI, H

TANAKA, N

UMEZAWA, H

机构：

[1] Laboratory of Microbiology, Department of Agricultural Chemistry, University of Tokyo, Tokyo, Bunkyo-ku

[2] Institute of Applied Microbiology, University of Tokyo, Tokyo, Bunkyo-ku

来源：

JOURNAL OF ANTIBIOTICS | 1969年 / 22卷 / 11期

关键词：

D O I：

10.7164/antibiotics.22.569

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Decrease of melting temperature (Tm) of Escherichia coli 15T-DNA, with little breakage of the DNA strand, was observed in the presence of bleomycin A2and 2-mercaptoethanol. DNA digested more drastically with pancreatic deoxyribonuclease (E. C. 3.1.4.5) revealed no shift of Tm. When the DNA, treated with bleomycin and 2-mercaptoethanol, was dialyzed after heat denaturation, marked strand scission occurred. Tm of poly (dG)•poly (dC) decreased by 9°C with a change of transition width at 40μg/ml of bleomycin, although that of poly d(AT)•poly d(TA) showed no change. Single strand scission of synthetic deoxyribopolymers was observed to occur slightly in the presence of bleomycin and markedly when dialyzed after the incubation. Strand scission without dialysis was produced more significantly on poly (dG)•poly (dC) than poly d(AT)•poly d(TA) in the presence of bleomycin. © 1969, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.

引用

页码：569 / &

共 13 条

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[2]

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[3]

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[4]

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