VARIABILITY IN RDNA LOCI IN THE GENUS ORYZA DETECTED THROUGH FLUORESCENCE IN-SITU HYBRIDIZATION

被引：163

作者：

FUKUI, K ^{[1
]}

OHMIDO, N ^{[1
]}

KHUSH, GS ^{[1
]}

机构：

[1] INT RICE RES INST, MANILA 1099, PHILIPPINES

来源：

THEORETICAL AND APPLIED GENETICS | 1994年 / 87卷 / 08期

关键词：

FLUORESCENCE IN-SITU HYBRIDIZATION (FISH); RIBOSOMAL DNA; GENUS ORYZA; IMAGE ANALYSIS; NOR VARIABILITY;

D O I：

10.1007/BF00225782

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The 17s-5.8s-25s ribosomal RNA gene (rDNA) loci in Oryza spp. were identified by the fluorescence in-situ hybridization (FISH) method. The rDNA loci were located on one-to-three chromosomes (two-to-six sites) within the eight diploid Oryza spp. One of the rDNA loci gave the weakest hybridization signal. This locus is reported for the first time in the genus Oryza. The chromosomes containing the rDNA loci were determined to be numbers 9, 10 and 11 in descending order of the copy number of rDNA. The application of image analysis methods, after slide preparation treatments (post-treatments), and the use of a thermal cycler, greatly improved the reproducibility of the results. The evolutionary significance of the variability of rDNA loci among the Oryza spp. is discussed.

引用

页码：893 / 899

页数：7

共 38 条

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