STEROID GLUCURONIDES - HUMAN CIRCULATORY LEVELS AND FORMATION BY LNCAP CELLS

被引：51

作者：

BELANGER, A ^{[1
]}

BROCHU, M ^{[1
]}

LACOSTE, D ^{[1
]}

NOEL, C ^{[1
]}

LABRIE, F ^{[1
]}

DUPONT, A ^{[1
]}

CUSAN, L ^{[1
]}

CARON, S ^{[1
]}

COUTURE, J ^{[1
]}

机构：

[1] LAVAL UNIV,QUEBEC CITY G1V 4G2,QUEBEC,CANADA

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1991年 / 40卷 / 4-6期

关键词：

D O I：

10.1016/0960-0760(91)90281-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the relationship between circulating androsterone glucuronide, androstane-3-alpha,17-beta-diol glucuronide and androstane-3-beta,17-beta-diol glucuronide concentrations and adrenal as well as testicular C-19 steroids in men. Among the three 5-alpha-reduced steroid glucuronides, androsterone glucuronide is the predominant C- 19 steroid glucuronide measured in plasma and its levels are markedly elevated compared to those of the non-conjugated steroid. The marked rise in testosterone during puberty was strongly correlated with the increase in both androsterone glucuronide and androstane-3-alpha, 17-beta-diol glucuronide, thus suggesting that testicular C-19 steroids are the main precursors of the steroid glucuronides. We also found that the presence of testicular androgen in plasma contributes to approx. 70% of plasma androsterone glucuronide and androstane-3-alpha, 17-beta-diol glucuronide. Our data suggest that the adrenal C-19 steroids remaining in circulation after castration in men are converted into potent androgen which are then glucuronidated by UDP-glucuronyltransferase. We also demonstrated that the human prostate cell line LNCaP is capable of converting to a large extent androstenedione into androsterone glucuronide. Our data further confirm that glucuronidation is a major pathway of steroid metabolism in steroid target tissues.

引用

页码：593 / 598

页数：6

共 14 条

[1] FORMATION OF GLUCURONIDES OF ESTRADIOL-17-BETA BY HUMAN MAMMARY-CANCER CELLS [J].