DRUG CYTOTOXICITY ASSAY FOR AFRICAN TRYPANOSOMES AND LEISHMANIA SPECIES

被引：91

作者：

BODLEY, AL ^{[1
]}

MCGARRY, MW ^{[1
]}

SHAPIRO, TA ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV CLIN PHARMACOL,BALTIMORE,MD 21205

来源：

JOURNAL OF INFECTIOUS DISEASES | 1995年 / 172卷 / 04期

关键词：

D O I：

10.1093/infdis/172.4.1157

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In this study, a spectrophotometric assay was developed and validated for measuring the cytotoxicity of test compounds against axenically cultured bloodstream-form Trypanosoma brucei (African trypanosomes) and promastigotes of Leishmania donovani. Enzymatic hydrolysis of p-nitrophenyl phosphate, monitored by a microtiter plate reader, is a reliable surrogate for parasite cell counts. The assay is simple, inexpensive, and highly reproducible. The coefficient of variation for ECS, values is <10% for determinations obtained over several months. This method permits the rapid screening of candidates for much-needed new drugs against these parasites.

引用

页码：1157 / 1159

页数：3

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