EXPRESSION OF THE ZYMOMONAS-MOBILIS ALCOHOL DEHYDROGENASE-II (ADHB) AND PYRUVATE DECARBOXYLASE (PDC) GENES IN BACILLUS

被引：28

作者：

BARBOSA, MDS ^{[1
]}

INGRAM, LO ^{[1
]}

机构：

[1] UNIV FLORIDA,DEPT MICROBIOL & CELL SCI,3095 MCCARTY HALL,GAINESVILLE,FL 32611

来源：

CURRENT MICROBIOLOGY | 1994年 / 28卷 / 05期

关键词：

D O I：

10.1007/BF01573206

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genes encoding Zymomonas mobilis pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were expressed in Bacillus subtilis YB886(pLOI500) under the control of a Bacillus SPO2 phage promoter and caused a 50% reduction of growth rate compared with the unmodified vector. Expression was further confirmed by Western blots, activity stains of native gels, and in vitro measurements of alcohol dehydrogenase activity. Additional strains of Bacillus were also transformed, and all produced similar but low levels of these enzymes. Although higher specific activities will be required for efficient ethanol production, no fundamental barriers exist to the expression of these Z. mobilis genes in Bacillus. Two abundant new proteins (ca. mass 33,000 daltons and 14,000 daltons) were observed in Coomassie Blue-stained gels; they are similar in size to the proteins induced by recombinant products in Escherichia coli.

引用

页码：279 / 282

页数：4

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