DETERMINATION OF ENKEPHALINS IN FETUS BRAIN TISSUES BY MEANS OF HPLC WITH PRE-COLUMN FLUORESCENCE DERIVATIZATION

被引：0

作者：

Zhao, Rui ^{[1
]}

Liu, Guo-quan ^{[1
]}

Feng, Fang-bo ^{[1
]}

Zhang, Ren-en ^{[1
]}

机构：

[1] Acad Sinica, Inst Chem, Beijing 100080, Peoples R China

来源：

ANALYTICAL SCIENCES | 1991年 / 7卷

关键词：

HPLC; FMOC-CL; fluorescence detection; enkephalin; human fetus brain;

D O I：

10.2116/analsci.7.Supple_951

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A HPLC method for the simultaneous determination of neuropeptides in complicated biological samples has been presented. 9-fluorenylmethyl chloroformate has been used as a pre-column fluorescent reagent. The linearity of this method is satisfactory in the range of 1-100 pmol. The concentrations of enkephalins in different regions of human fetus brain has been determined using HPLC method and fell in the range of several to hundreds pmol per gram wet tissue.

引用

页码：951 / 954

页数：4

共 19 条

[1] A SENSITIVE COUPLED HPLC-RIA TECHNIQUE FOR SEPARATION OF ENDORPHINS - MULTIPLE FORMS OF B-ENDORPHIN IN RAT PITUITARY INTERMEDIATE VS ANTERIOR LOBE [J].

AKIL, H ;

UEDA, Y ;

LIN, HL ;

WATSON, SJ .

NEUROPEPTIDES, 1981, 1 (06) :429-446

[2]

Berritteni W. H., 1982, LIFE SCI, V31, P1

[3] IMPROVEMENT IN METHODOLOGY FOR MEASUREMENT OF PLASMA RENIN ACTIVITY [J].

BOUCHER, R ;

GENEST, J .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1966, 44 (01) :181-&

[4] ENKEPHALIN - RADIOIMMUNOASSAY AND RADIORECEPTOR ASSAY IN MORPHINE-DEPENDENT RATS [J].

CHILDERS, SR ;

SIMANTOV, R ;

SNYDER, SH .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1977, 46 (03) :289-293

[5] DETERMINATION OF AMINO-ACIDS WITH 9-FLUORENYLMETHYL CHLOROFORMATE AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

EINARSSON, S ;

JOSEFSSON, B ;

LAGERKVIST, S .

JOURNAL OF CHROMATOGRAPHY, 1983, 282 (DEC) :609-618

[6] SIMULTANEOUS ANALYSIS OF METHIONINE-ENKEPHALIN AND LEUCINE-ENKEPHALIN FROM RAT-BRAIN - QUANTIFICATION BY LIQUID-CHROMATOGRAPHY - ELECTROCHEMISTRY [J].

FLEMING, LH ;

REYNOLDS, NC .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 431 (01) :65-76

[7]

Hong J. S., METHODS ENZYMOLOGY, V103, P552

[8] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF TYROSINE-CONTAINING PEPTIDES BY PRECOLUMN DERIVATIZATION INVOLVING FORMYLATION FOLLOWED BY FLUORESCENCE REACTION WITH 1,2-DIAMINO-4,5-DIMETHOXYBENZENE [J].

ISHIDA, J ;

KAI, M ;

OHKURA, Y .

JOURNAL OF CHROMATOGRAPHY, 1986, 356 (01) :171-177

[9] USE OF REVERSED-PHASE AND ION-EXCHANGE BATCH EXTRACTION IN THE PURIFICATION OF BOVINE PITUITARY PEPTIDES [J].

JAMES, S ;

BENNETT, HPJ .

JOURNAL OF CHROMATOGRAPHY, 1985, 326 (JUN) :329-338

[10] DETERMINATION OF LEUCINE-ENKEPHALINS AND METHIONINE-ENKEPHALINS IN RAT BRAINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PRECOLUMN FLUORESCENCE DERIVATIZATION [J].

KAI, M ;

NAKANO, M ;

ZHANG, GQ ;

OHKURA, Y .

ANALYTICAL SCIENCES, 1989, 5 (03) :289-293

← 1 2 →