CLEAVAGE MAP OF THE TOL PLASMID OF PSEUDOMONAS-PUTIDA MT-2

被引：40

作者：

DOWNING, R

BRODA, P

机构：

[1] Department of Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR, Kings Buildings

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 177卷 / 01期

关键词：

D O I：

10.1007/BF00267270

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants. © 1979 Springer-Verlag.

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页码：189 / 191

页数：3

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